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PCR Cloning Service

GW Vitek (Ltd.) offers PCR cloning service that moves various DNA fragment with the vector that researchers desire. Plasmid DNA, cDNA are sample for PCR. Service period of PCR cloning service depends on template size needed to be amplified or template chances.

We guarantee 100% same sequence with template sequence that researchers provide. PCR cloning service is confirmed to be a clone with the same sequence of template through sequencing finally.

Subcloning Service

GW Vitek (Ltd.) offer subcloning service that alters vector using restriction enzymes aside from PCR cloning. Subcloning service has no difference in its service period depending on template size unlike PCT cloning and service period is about 2 weeks. It is the service that only moves template within plasmid that researchers provide and sequencing after subcloning is not included in the service.


PCR cloning Service Protocol


Subcloning Service Protocol


Service Details

ServiceResearcher providesGW Vitek (Ltd.) provides
PCR Cloning

Usable Template

  • cDNA 20 ㎕
  • PCR Product 100 ㎕ (0.1 ㎕/㎍)
  • Template plasmid DNA 10 ㎕ (0.1 ㎍/㎕)

Service commence

  • Info-sheet

Service information

  • Destination vector
    (vector’s name, antibiotics, enzyme site, etc.)
  • Template vector
    (vector’s name, antibiotics, enzyme site, etc.)
  • Template information
    (Gene-sequence, NCBI No, etc.)

Service complete

  • Result report
  • Cloned plasmid
  • Cell stock (E.coli strain: DH5α)
  • Primer pair, rest of sample by researchers supply
Subcloning

Usable Template

  • Plasmid DNA 20 ㎕ (0.1 ㎍/㎕)

Service commence

  • Info-sheet

Service Information

  • Destination vector
    (vector’s name, antibiotics, enzyme site, etc.)
  • Template vector
    (vector’s name, antibiotics, enzyme site, etc.)
  • Template information
    (Gene-sequence, NCBI No, etc.)

Service complete

  • Result report
  • Subcloned plasmid
  • Cell stock (E.coli strain: DH5α)
  • Rest of sample by researchers supply
  • researchers could use own vectors or select GW Vitek’s for PCR cloning and subcloning. (Need to inquire for the retention of vectors)