Product Name :
Hsp27, Human
Product Type :
Protein
Lot No :
QJN02
Reactivity :
Human
Storage :
Shipped at 4°C. Store at -20°C. After reconstitution, store at -80°C and avoid frequent freeze and thaw.
Composition :
Lyophilized from 50mM NaH2PO4, pH8.0 / 300mM NaCl / 250mM imidazole
Source :
Purified from E.coli expressing the human Hsp27 (1-205aa, with N-His tag)
Molecular Weight :
25 kDa
Endotoxin activity :
Not tested
Usage :
200ul of distilled water will yield a concentration of 1mg/ml.
Form :
Lyophilized
Purity :
> 95% by SDS-PAGE
Expression system :
E.coli
Tag :
His tag
Origin :
Human
Background :
Heat shock proteins are ubiquitous proteins and have been characterized as cytoprotective molecular chaperones. The typical function of a chaperone is to assist a protein to attain its functional conformation to prevent non-functional aggregation of misfolded proteins. The principal HSP families are HSP90, HSP70, HSP60 and the small HSPs including HSP27, ubiquitin, α-crystallin, Hsp20 and others. The common functions of small Hsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. Hsp27 has a molecular weight of approximately 27 kDa, although it has been shown to form large aggregates of up to 800 kDa in the cytosol. Hsp27 is found in several types of human cells, including tumour cells. Hsp27 interferes with apoptosis through its ability to interact with and inhibit key components of the apoptotic signaling pathway, including the caspase activation complex. Overexpression of heat shock proteins can increase the tumorigenic potential of tumour cells. HSP27 also has been reported to be involved in development and progression of hormone-refractory prostate cancer.
Background reference :
1) So A et al., 2007, Curr Genomics 8(4):252-261
2) Ferns G et al., 2006, Int J Exp Pathol 87(4):253-274
3) Ciocca DR and Calderwood SK, 2005, Cell Stress Chaperones 10(2):86-103
Research area :
Signal Transduction 
Database link - SwissProt no.
P04792

The test was performed by customers with their own sample.