New Antibodies from Gentaur


  1. 11-931-C100 Anti-Hu CD368 Purified CD368 Non-human primates, Human 9B9 Purified RUO Mouse IgG2b kappa ICC, FC (QC tested), ELISA, IP CD368 ectodomain fused with human Fc The mouse monoclonal antibody 9B9 recognizes an extracellular epitope of CD368, a type II transmembrane protein of C lectin family, expressed mainly on monocytes and neutrophils. 0.1 mg 1 mg/ml Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Store at 2-8°C. Do not freeze. CD368 is an approximately 25-30 kDa C-type lectin, which serves as one of pattern recognition receptors of the innate immune system. It recognizes pathogen-associated molecular patterns of bacteria and fungi, such as alpha-mannans or trehalose 6,6´-dimycolate, and transfers the signal downstream using associated Fc receptor gamma chain. CD368 is expressed on neutrophils, monocytes, and on some populations of blood dendritic cells, but it decreases during differentiation of monocytes into dendritic cells or macrophages. Increased expression can be induced by IFN-gamma, TNF-alpha, IL-6, and IL-10. CD368 triggering leads to driving of antigen-presenting cells maturation and to promotion of T cell differentiation into Th1 and Th17. Flow cytometry: Recommended dilution: 1-5 µg/ml *Damasceno D, Andrés MP, van den Bossche WB, Flores-Montero J, de Bruin S, Teodosio C, van Dongen JJ, Orfao A, Almeida J: Expression profile of novel cell surface molecules on different subsets of human peripheral blood antigen-presenting cells. Clin Transl Immunology 2016 Sep 16;5(9):e100., URL: https://pubmed.ncbi.nlm.nih.gov/27766148/,*Autenrieth SE, Grimm S, Rittig SM, Grünebach F, Gouttefangeas C, Bühring HJ: Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 workshop in Wollongong, Australia. Clin Transl Immunology 2015 Nov 13;4(11):e50. , URL: https://pubmed.ncbi.nlm.nih.gov/26682057/,*Clark G, Stockinger H, Balderas R, van Zelm MC, Zola H, Hart D, Engel P: Nomenclature of CD molecules from the Tenth human leucocyte differentiation antigen workshop. Clin Transl Immunology 2016 Jan 22;5(1):e57., URL: https://pubmed.ncbi.nlm.nih.gov/26900471/,

  2. 11-937-C100 Anti-Hu IL-17A Purified IL-17A Human 9F9 Purified RUO Mouse IgG1 kappa IP, ELISA, FC (QC tested), ICC mammalian-derived human IL-17-IgG fusion protein, boost with recombinant human IL-17A The mouse monoclonal antibody 9F9 recognizes human interleukin 17A (IL-17A; secreted or intracellular). 0.1 mg 1 mg/ml Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Store at 2-8°C. Do not freeze. Interleukin 17A (IL-17A) is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. It plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. It is the signature effector cytokine of Th17 cells, and in this role it primarily induces neutrophil activation and recruitment at infection and inflammatory sites. High levels of IL-17A are associated with rheumatoid arthritis, psoriasis, multiple sclerosis, and another inflammatory diseases, including lung injugy during severe COVID 19. This cytokine also contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. It is an effector cytokine for invariant NKT cells (iNKT), and it is involved in epithelial barrier formation upon injury. Flow cytometry: Recommended dilution: 0.5-4 µg/ml. Intracellular staining.

  3. 11-936-C100 Anti-Hu IL-2 Purified IL-2 Human 35C3 Purified RUO Mouse IgG2b kappa IP, FC (QC tested), ELISA, ICC, WB recombinant human IL-2 The mouse monoclonal antibody 35C3 recognizes human interleukin 2 (IL-2; secreted or intracellular). 0.1 mg 1 mg/ml Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Store at 2-8°C. Do not freeze. IL-2 (interleukin 2) is a cytokine that is produced primarily by stimulated Th cells and its crucial role is induction of T cell proliferation. However, IL-2 also stimulates growth and differentiation of B cells, NK cells, monocytes and other cell types, such as LAK cells or oligodendrocytes and is one of the key molecules of the immune system. IL-2 signaling pathways lead to induction of Bcl-2 protein. Flow cytometry: Recommended dilution: 0.5-4 µg/ml. Intracellular staining.

  4. A6-438-C100 Anti-HLA-G Alexa Fluor<sup>®</sup> 647 HLA-G Human Mouse, Rat 01G Alexa Fluor<sup>®</sup> 647 RUO Mouse IgG1 IHC(F), FC (QC tested), ICC HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin. The antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. 0.1 mg 1 mg/ml Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage. Flow cytometry: Recommended dilution: 2-5 μg/ml. *Polakova K, Russ G: Expression of the non-classical HLA-G antigen in tumor cell lines is extremely restricted. Neoplasma. 2000;47(6):342-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/11263857?dopt=,*Polakova K, Bandzuchova E, Hofmeister V, Weiss EH, Hutter H, Russ G: Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients. Neoplasma. 2003;50(5):331-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/14628085?dopt=,*Polakova K, Krcova M, Kuba D, Russ G: Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients. Leuk Res. 2003 Jul;27(7):643-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/12681364?dopt=,*Wiendl H, Feger U, Mittelbronn M, Jack C, Schreiner B, Stadelmann C, Antel J, Brueck W, Meyermann R, Bar-Or A, Kieseier BC, Weller M: Expression of the immune-tolerogenic major histocompatibility molecule HLA-G in multiple sclerosis: implications for CNS immunity. Brain. 2005 Nov;128(Pt 11):2689-2704., URL: https://pubmed.ncbi.nlm.nih.gov/16123145/,*Real LM, Cabrera T, Collado A, Jimenez P, Garcia A, Ruiz-Cabello F, Garrido F: Expression of HLA G in human tumors is not a frequent event. Int J Cancer. 1999 May 17;81(4):512-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/10225437?dopt=,*Real LM, Cabrera T, Canton J, Oliva R, Ruiz-Cabello F, Garrido F: Looking for HLA-G expression in human tumours. J Reprod Immunol. 1999 Jul;43(2):263-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/10479062?dopt=,*Aractingi S, Briand N, Le Danff C, Viguier M, Bachelez H, Michel L, Dubertret L, Carosella ED: HLA-G and NK receptor are expressed in psoriatic skin: A possible pathway for regulating infiltrating T cells? Am J Pathol. 2001 Jul;159(1):71-7., URL: https://pubmed.ncbi.nlm.nih.gov/11438456/,

  5. A6-924-T100 Anti-Hu CD1d Alexa Fluor<sup>®</sup> 647 CD1d Human, Non-human primates 51.1 Alexa Fluor<sup>®</sup> 647 RUO Mouse IgG2b kappa IHC(F), WB, FC (QC tested) human CD1d The mouse monoclonal antibody 51.1 recognizes an extracellular epitope of CD1d, a 38 kDa transmembrane glycoprotein expressed mainly on cortical thymocytes, marginal zone B cells and other antigen presenting cells, but also i e.g. hepatitis C virus-infected livers. 100 tests Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. CD1d belongs to CD1 family of transmembrane glycoproteins, associated with beta2 microglobulin, similarly to MHC I molecules. Unlike other CD1 family members, however, CD1d can be also expressed in a non-glycosylated form, which is not associated with beta2 microglobulin. Hence it is not certain how much CD1d plays a role in the presentation of microbial lipid antigens during infection. On the other hand, it is expressed on various antigen presenting cell types. Besides it, CD1d+ thymocytes are involved in the positive selection of sublineage of NKT cells. Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. *Kim HS, Garcia J, Exley M, Johnson KW, Balk SP, Blumberg RS: Biochemical characterization of CD1d expression in the absence of beta2-microglobulin. J Biol Chem. 1999 Apr 2;274(14):9289-95., URL: https://pubmed.ncbi.nlm.nih.gov/10092605/,*Durante-Mangoni E, Wang RJ, Shaulov A, He Q, Nasser I, Afdhal N, Koziel MJ, Exley MA: Hepatic CD1d expression in hepatitis C virus infection and recognition by resident proinflammatory CD1d-reactive T cells. J Immunol. 2004 Aug 1;173(3):2159-66., URL: https://pubmed.ncbi.nlm.nih.gov/15265953/,

  6. A6-292-C100 Anti-HLA-G Alexa Fluor<sup>®</sup> 647 HLA-G Human Mouse MEM-G/9 Alexa Fluor<sup>®</sup> 647 RUO Mouse IgG1 IHC(F), ICC, FC (QC tested) Recombinant human HLA-G refolded with beta2-microglobulin and peptide. The antibody MEM-G/9 reacts with an extracellular epitope on native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003). 0.1 mg 1 mg/ml Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. Human leukocyte antigen G (HLA-G), belonging to MHC class I glycoproteins, plays important roles in both physiological and pathological immunotolerance. It gives an inhibitory signal to cytotoxic T cells, NK cells, monocytes, and some other immune cells. It also induces regulatory T cells and anti-inflammatory macrophages. HLA-G is important e.g. for maternal tolerance to the fetus, and for immunomodulation in particular adult tissues, such as in cornea, pancreatic islets, thymus and other. On the other hand, it is expressed in many solid and hematologic malignancies, where it contributes to evasion of the immune surveillance. HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome. Unlike most other MHC glycoproteins, HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule. It concerns both transmembrane and soluble HLA-G isoforms. Among other, HLA-G can promote Th2 immunological response and downregulate Th1 immunological response. For its benefits regarding allograft tolerance, including embryo implantation, soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization. Similarly, sHLA-G concentrations in maternal serum are decreased in preeclampsia. Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance. On the other hand, increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells. Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies, which can weaken the therapy effect. Monitoring of HLA-G in patients thus has a wide usage. Flow cytometry: Recommended dilution: 1-4 µg/ml *Zhao L, Teklemariam T, Hantash BM: Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies. Tissue Antigens. 2012 Sep;80(3):231-8, URL: http://www.ncbi.nlm.nih.gov/pubmed/22738368 ,*Fuzzi B, Rizzo R, Criscuoli L, Noci I, Melchiorri L, Scarselli B, Bencini E, Menicucci A, Baricordi OR: HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. Eur J Immunol. 2002 Feb;32(2):311-5. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11807769?dopt=,*Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules. J Immunol. 2005 Oct 15;175(8):4866-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/16210588?ordin,*Lopez AS, Alegre E, LeMaoult J, Carosella E, Gonzalez A. Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells. Mol Immunol. 2006 Jul;43(14):2151-60., URL: https://www.ncbi.nlm.nih.gov/pubmed/16490253?dopt=,*Abstracts from the 3rd International Conference on HLA-G. Tissue Antigens 62, 339-357 (2003)., URL: ,*Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/12590976?dopt=,*Pangault C, Le Friec G, Caulet-Maugendre S, Lena H, Amiot L, Guilloux V, Onno M, Fauchet R: Lung macrophages and dendritic cells express HLA-G molecules in pulmonary diseases. Hum Immunol. 2002 Feb;63(2):83-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/11821155?dopt=,*Lozano JM, Gonzalez R, Kindelan JM, Rouas-Freiss N, Caballos R, Dausset J, Carosella ED, Pena J: Monocytes and T lymphocytes in HIV-1-positive patients express HLA-G molecule. AIDS. 2002 Feb 15;16(3):347-51., URL: https://www.ncbi.nlm.nih.gov/pubmed/11834945?dopt=,*Fournel S, Huc X, Aguerre-Girr M, Solier C, Legros M, Praud-Brethenou C, Moussa M, Chaouat G, Berrebi A, Bensussan A, Lenfant F, Le Bouteiller P: Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules. Tissue Antigens. 2000 Jun;55(6):510-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/10902607?dopt=,*Morandi F, Ferretti E, Bocca P, Prigione I, Raffaghello L, Pistoia V: A novel mechanism of soluble HLA-G mediated immune modulation: downregulation of T cell chemokine receptor expression and impairment of chemotaxis. PLoS One. 2010 Jul 23;5(7):e11763. , URL: https://www.ncbi.nlm.nih.gov/pubmed/20668702,*Emadi E, Khoradmehr A, Shams A, Kalantar SM: Appropriate fixative for MEM-G/9 staining of cultured human HLA-G-positive JEG-3 trophoblast tumor cells. Biotech Histochem. 2022 Feb;97(2):136-142., URL: https://pubmed.ncbi.nlm.nih.gov/33910426/,

  7. 1B-166-C100 Anti-Cyclin D1 Biotin Cyclin D1 Rat, Mouse, Non-Human Primates, Human Cat, Sheep DCS-6 Biotin RUO Mouse IgG2a IHC(F), FC, IP, ICC, IHC(P), WB recombinant human cyclin D1 (amino acids 1-295) The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions. 0.1 mg 1 mg/ml Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Store at 2-8°C. Do not freeze. Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. Flow cytometry: Recommended dilution: 2-10 μg/ml. Intracellular staining.<br>Immunohistochemistry: Recommended dilution: 5-10 μg/ml. *Bosone I, Cavalla P, Chiad&#242;-Piat L, Vito ND, Schiffer D: Cyclin D1 expression in normal oligodendroglia and microglia cells: its use in the differential diagnosis of oligodendrogliomas. Neuropathology. 2001 Sep;21(3):155-61. , URL: http://www.ncbi.nlm.nih.gov/pubmed/11666011 ,*Jain P, Giustolisi GM, Atkinson S, Elnenaei MO, Morilla R, Owusu-Ankomah K, Rafiq-Mohammed F, Matutes E, Wotherspoon A, Catovsky D: Detection of cyclin D1 in B cell lymphoproliferative disorders by flow cytometry. J Clin Pathol. 2002 Dec;55(12):940-5., URL: http://www.ncbi.nlm.nih.gov/pubmed/12461064 ,*Albrecht JH, Hansen LK: Cyclin D1 promotes mitogen-independent cell cycle progression in hepatocytes. Cell Growth Differ. 1999 Jun;10(6):397-404., URL: http://www.ncbi.nlm.nih.gov/pubmed/10392901 ,*Zwijsen RM, Buckle RS, Hijmans EM, Loomans CJ, Bernards R: Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1. Genes Dev. 1998 Nov 15;12(22):3488-98., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=Zwijsen+rm+buckle+rs+bernards+r ,*Fredersdorf S, Burns J, Milne AM, Packham G, Fallis L, Gillett CE, Royds JA, Peston D, Hall PA, Hanby AM, Barnes DM, Shousha S, O'Hare MJ, Lu X: High level expression of p27(kip1) and cyclin D1 in some human breast cancer cells: inverse correlation between the expression of p27(kip1) and degree of malignancy in human breast and colorectal cancers. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6380-5., URL: https://pubmed.ncbi.nlm.nih.gov/9177226/,

  8. 1P-859-T100 Anti-Hu CD307b PE CD307b Human B24 PE RUO Mouse IgG2a kappa FC (QC tested) DNA-immunization followed by a boost with CD307b transfected cells The mouse monoclonal antibody B24 recognizes an extracellular epitope of CD307b, a transmembrane glycoprotein expressed mainly in mature and memory B cells. 100 tests Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. CD307b is a type I transmembrane glycoprotein of the Fc receptor family. It contains one ITAM motif and two ITIM motifs in its cytoplasmic domain. It is expressed in spleen and lymph nodes in mature B cells and memory B cells. CD307b may be a prognostic marker for chronic lymphocytic leukemia. Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. *Nagata S, Ise T, Pastan I: Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells. J Immunol. 2009 Jun 15;182(12):7518-26, URL: https://www.ncbi.nlm.nih.gov/pubmed/19494275,

  9. 1F-765-T100 Anti-Perforin FITC Perforin Human, Cow Mouse dG9 FITC RUO Mouse IgG2b kappa FC (QC tested) purified granules from human YT lymphoma cell line The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells. 100 tests Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders. Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining. *Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8&#945;(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011 Nov;134(3):326-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/21978002,*Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010 Jun;2(3):139-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/20348186 ,*Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions. PLoS Pathog. 2012;8(11):e1002984., URL: http://www.ncbi.nlm.nih.gov/pubmed/23144609 ,*Hersperger AR, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov;73(11):1050-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18615597 ,*Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER: Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May;140(5):1025-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/1374586 ,

  10. 1P-425-C100 Anti-DDDDK tag PE DDDDK tag Tagged fusion proteins in all species F-tag-01 PE RUO Mouse IgG1 ICC Synthetic peptide: DYKDDDDK conjugated to KLH The mouse monoclonal antibody F-tag-01 recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function. 0.1 mg 0.1 mg/ml Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. *Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37., URL: https://www.ncbi.nlm.nih.gov/pubmed/16724116?dopt=,*Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of print], URL: https://www.ncbi.nlm.nih.gov/pubmed/19304756?ordin,


Internal Reference: YF-PA26408