BD FACSymphony™ S6 Cell SorterSpecial Order Research ProductA cell sorter to put you at the forefront of research

High parameter flow cytometry has been a primary driver in recentbiological research. And cell sorters have not kept up with therequirements for isolating newly discovered subpopulations. Untilnow. Today the ability to sort distinct phenotypes is available foryour lab. What’s the first experiment you would do with the abilityto sort based on high parameter flow cytometry?

The BD FACSymphony S6 cell sorter is designed to power yourscientific discoveries – the cutting edge discoveries that will definea new future in your field and have a lasting impact.Cutting edge science requires cutting edge technology and theBD FACSymphony S6 delivers.Built on the solid foundations of the BD FACSymphony™ A5 cellanalyzer and BD FACSAria™ Fusion cell sorter, the innovations in theBD FACSymphony S6 bring cell sorting in line with the capabilitiesof modern flow cytometry. Now, you can sort populations identifiedwith 20 or more colors in the complex hierarchies required for yourapplication.But more than this, when coupled with BD’s broad portfolio ofhigh parameter reagents, bioinformatics analysis tools, single cellmultiomics solutions and applications support capabilities, the BDFACSymphony S6 equips researchers with a complete, end-to-endsolution for your high parameter experimental success.Spearheading New DiscoveriesEnable the sorting of ultra-specific populations by using up to 60parameters to define populations deep in your hierarchy and sort upto 6 of them at one time so that you can get more information fromeach cell and more value from each experiment – whether it’s moreprecisely isolating the population of interest prior to your downstreamassay, or linking deeper phenotypic information with your functionalor genomic read-outs. All this data puts you on the right track to resultswhich make an impact.But the data set is not just powerful because there's more informationper cell, it's high quality data as well. Low-noise electronics and highly-sensitive PMT detectors tuned to the appropriate detector wavelengthsare combined with optics designed to maximize signal collection,including the BD patented gel-coupled cuvette, in order to provideexcellent sensitivity to resolve dim populations and explore thenuances of marker expression deep within the biology of the sample. BD FACS™ Accudrop technology enables high-performance sortresults by maintaining an optimal breakoff and pausing the sortwhen necessary to protect the purity of the sorted sample.Index sorting makes it possible to review the cell-surface phenotype ofevery cell deposited into a multi-position device, such as a 96-wellplate. Sort and tray position information is available on an event-by-event basis in order to correlate post-isolation results to eachindividual sorted cell, allowing you to link a precise phenotype toyour downstream experimental observations.

Accelerating scienceLeveraging the leadership of BD across high parameter flow cytometryBD's innovations in high parameter instrumentation with the BD FACSymphony analyzers and in high parameter reagents with the BDHorizon Brilliant™ Dyes are the natural foundation for a high parameter cell sorter. The combination of all three – dyes, analyzers andsorter – leads to the broadest solution for high parameter experimentation available, enabling you to move quickly from biologicalquestion and analysis to sorting.Designing panels takes time and reagents away from the discoveries of an experiment and has previously been a bottle neck in movingfrom analysis to sorting. Now, with the ability to match your configurations between your BD FACSymphony A5 analyzer and your BDFACSymphony S6 cell sorter, you can easily transfer a panel from one to the other without spending the time to develop a new panel.Even if 20 parameters are not required to sort your subpopulations, you can rest easy knowing that the fluorochromes paired with thekey markers of interest are available on the BD FACSymphony S6.Accelerate your discoveries by taking a panel you’ve optimized on your BD FACSymphony A5 and move it to the BD FACSymphony S6to sort, with more parameters than any other analyzer and sorter combination.Figure 1: A 27-color panel for analysis of immune cell composition in miceA 27-color mouse panel was optimized on the BD FACSymphony A5 and target MFIs were transferred to the BD FACSymphony S6 to allow sorting 6 distinct subsets from the panel:Naive CD8 T Cells, Mantle Zone B Cells, IgD+ CD21+ B Cells, CD11b Hi Natural Killer Cells, CD11b Lo Natural Killer Cells, and CD127+CD27⁻ T Cells. Cells were gated on scatter, as well asdoublets and dead cells (7-AAD positive) were excluded (data not shown) prior to gating for the sort populations above. Of particular interest, the CD127+CD27⁻sort population was inthe 10th generation of the gating hierarchy. All populations were between 1 and 10% of live cells, and achieved greater than 90% purity (data not shown).Figure 1CD19APC-H7CD5 PECD8aBUV395CD4 BV570NK1.1BB790CD3ε APCLy6CBUV737CD62L BB515CD314/NKG2D BUV615CD62L BB515CD19APC-H7CD45R/B220 BUV805CD21BUV661CD23 BV650CD93PE-CF594CD45R/B220 BUV805CD21BUV661IgD BUV496CD27BV711CD11b APC-R700CD3εAPCNK1.1 BB790Ly6CBUV737CD44BV421CD27BV711CD127 BB660CD5+ CD19⁻CD3+ NK1.1⁻NK1.1+ CD3⁻CD44+ CD62L⁻CD11blow SortCD11bhigh SortCD127+CD27⁻SortB CellsMZ B Cells SortIgD+ CD21+ SortFO B CellsMatureLy6C⁻ NKG2D⁻CD8a+Naïve CD8 SortCD4+CD5⁻CD19⁻CD5⁻CD19⁻CD4+CD8a+

Flexible PlatformBuilt with the flexibility of BD's Special Order Research Product (SORP) program, theBD FACSymphony S6 is designed to meet your assay’s needs – no matter how unique.• The system can be configured with up to 9 spatially separated lasers. There are more than25 wavelength options available through SORP.• New high parameter cascades allow up to 20 parameters on a single laser line,without losing sensitivity and use removable filters that can be completelycustomized for optimal detection of dyes andfluorescent proteins.• A multitude of collection devices are provided with new flexibilityto change tubes and holders without stopping the sort.• High-precision interchangeable nozzles are available for a wide rangeof cell size support: 70, 85, and 100 µm come standard. An optional130 um nozzle is also available.• Have peace of mind through support for field upgrades, including theaddition of new laser lines or additional parameters in the future, andthe biosafety cabinet after purchase.• Contact your local BD Representative for a full discussion on optionswith the BD FACSymphony S6.Figure 2405980808785752730685660647640637594588561552568532520514505458445422413375360355349488473mWmW100200300400500BD FACSymphonyPatented detector technology allows for up to20 parameters on one laser line.Figure 2: Available laser wavelength and power options through the Special Order Research ProgramEach ray of the diagram represents an available laser wavelength, and each segment represents an available laser power of the given wavelength. Please contact your BD representativefor a full list of laser options.

Priority on BiosafetyBD and The Baker Company have joined forces to provide a biosafety solution whichmeets personnel and product protection standards.BD knows that your safety is important so the BD FACSymphony S6 has been designed with safety in mind. The purpose-built BDFACSAria Fusion biosafety cabinet (BSC) with independent aerosol management system (AMS) means that National SanitationFoundation International Standard 49, the European Standard 12469, the China Standard SFDA YY- 0569:2005 and the AustralianStandard AS 2252.2– 2009 for personnel and product protection are all met.Since the BD FACSymphony S6 and The Baker Company BSC were designed for a seamless fit, a BSC can be added later in a field upgradethat takes less than a day to install.Additionally, the easy-to-clean stainless steel sort chamber, enclosed sample pathway and the Prepare for Aseptic Sort procedure allenable you to prioritize biosafety while also prioritizing performance and usability with the BD FACSymphony S6.

Share of Flow Cytometry Citations*BD BiosciencesAll other companies92.82%Built by BD, as part of the BD Total SolutionBuilding on our 40-year history of expertise and innovation in flow cytometry, it only makes sense that the game-changing BD FACSymphony S6comes from BD. BD has consistently led the progression to higher parameter flow cytometry with instruments and reagents and we continuethat progression now with high parameter cell sorting.BD has led the way with thousands of peer-reviewed publications usingBD instruments, technology and software. The BD FACSymphony S6is built on that legacy with the reliability and performance of theBDFACSAria Fusion instrument, and the cutting-edge optics andelectronics of the BD FACSymphony A5. With a familiar interface viathe BD FACSDiva software package, users will quickly be up to speedon running the BD FACSymphony S6, but be appreciative of thereengineering to take full advantage of Windows 10 64-bit power.SupportIn addition to innovative technology and a history of reliability, BD has a worldwide team of engineers and application specialists ready to helpcustomers through comprehensive training and field service calls. Hands-on training is included with each BD FACSymphony S6 cell sorter,and applications specialists are available to help with panel design.ReagentsOur broad portfolio of fluorochromes featuring the BD Horizon Brilliant dyes and BD OptiBuild™ technology, along with our custom reagentservices, offer flexibility for experimental design. BD Horizon Brilliant dyes were developed utilizing Nobel-prize winning Sirigen technology to expand the number and options offluorochromes for the blue, yellow-green, violet, and UV lasers. These dyes were selected for their specific spectral characteristics such asincreased fluorescence intensity, reduced cross-laser excitation and reduced spillover into other channels.FlowJo™Utilize FlowJo software for your high-parameter analysis needs.In addition to native models for advanced data analysis andnecessary tools for publication-quality graphics, FlowJo v10software has a plug-in architecture to enable access to the latestalgorithms and easiest workflows. Popular plug-ins from FlowAI toFlowSOM are available to help you get the most out of your high-parameter data, and now, the HyperFinder plugin and theFACSDiva™ import and export workflow combine to allow you todefine a gating strategy for a population found using anycombination of clustering, gating and dimensionality reduction.Single Cell MultiomicsA cell sorter is often the gateway to downstream single cell applications and in this regard, the BD FACSymphony S6 combined with the BDRhapsody™ Single-Cell Analysis System make a powerful pair. Utilize the FACSymphony to target and isolate specific, rare cell populationsthat require high parameter sorting. Make great discoveries and gain novel insights about the sorted populations via multiomic analysesperformed using BD® AbSeq and the BD Rhapsody Single-Cell Analysis System. Combining sorting with single cell analyses also dramaticallylowers the sequencing costs of your experiments.*Global citations from 1988-2017.Data provided by CiteAb.

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