Unlike the traditional protein gel preparation and precast gel, this product on/y needs praparation and potting of separation gel and stacking gel fo complets protain gel in 20 min and profein e/ectrophoresis in 30 min. (t has low acryfamide concenrrarion needs no TEMED, and thus is safcc.

Dengue Duo Cassette
R-DEN03D
The Panbio Dengue Duo Cassette is a rapid immunochromatography test designed for the qualitative presumptive detection of IgM and IgG antibodies to dengue virus in human serum, plasma and whole blood.

Dengue IgG Indirect ELISA
E-DEN01G
The Panbio Dengue IgG Indirect ELISA is for the qualitative detection of IgG antibodies to dengue antigen in serum, as an aid in the clinical laboratory diagnosis of patients with clinical symptoms and past exposure consistent with dengue fever.

Dengue Duo IgM & IgG Capture ELISA
E-DEN01D
Please note these products are not for sale or distribution in the United States and may not be available in all countries worldwide. Please contact Inverness Medical or your local Panbio distributor to determine availability.


Product Overview
The Panbio Dengue Duo IgM Capture & IgG Capture ELISA is for the qualitative detection of IgM and IgG antibodies to dengue virus in serum.

The Panbio Dengue Duo IgM Capture & IgG Capture ELISA offers an improvement over the HAI assay as an aid for the serological diagnosis of dengue infections between primary and secondary infection using a single dilution of serum, rather than a series of dilutions required for HAI assays.

The IgM cut-off is set to detect IgM levels characteristic of primary infection.

These ELISA tests are designed to distinguish between primary and secondary infections
This kit consists of the Dengue IgM Capture ELISA and the Dengue IgG Capture ELISA packaged together for convenience and cost savings
Ideal for use in regions where primary and secondary dengue are common
For the differentiation of primary and secondary dengue
Quick Info
Standardised method
Short incubation time (2 hr 10 min)
Break-apart wells
Ready-to-use colour coded reagents
Compatible with standard microplate technology
Reliable performance
Dengue IgM Capture and Dengue IgG Capture ELISA kits packaged together (192 tests)
Differentiates between primary and secondary infection
Performance
Dengue IgM Capture and Dengue IgG Capture ELISA serological sensitivity and specificity1
Serological sensitivity IgM 95% CI* IgG 95% CI*
Primary dengue
54/57 (94.7%)
85.4-98.91%
N/A
N/A
Secondary dengue2 64/115 (55.7%)3 46.6-64.7% 52/54 (96.3%) 73.8-93.6%
Serological specificity IgM 95% CI* IgG 95% CI
Negatives
83/83 (100%)
95.7 - 100 % 125/125 (100%) 97.1-100%
Primary dengue N/A 95.7 - 100 % 32/35 (91.4%) 76.9-98.2%
This data has been extracted from the Dengue IgM Capture and Dengue IgG Capture ELISA Instructions for Use.
Samples positive for secondary dengue infection by HAI (titre >= 2560)
The use of the Panbio Dengue IgG Capture ELISA (E-DEN02G) is recommended when secondary dengue is suspected
* Confidence Interval

Cross-reactivity data - Panbio Dengue IgM Capture ELISA1
Disease type Total specimens Positive (or equivocal) result
Epstein-Barr virus 10 0/10
Malaria 10 1/10
Influenza A 7 0/7
Influenza B 3 0/3
Anti-nuclear antibody 30 0/30
Rheumatoid factor 10 3/10
Hepatitis A 9 0/9
Leptospira 7 0/7
Salmonella typhi 9 0/9
Scrub typhus 10 0/10
West Nile virus 10 2/10
TOTAL 115 6/115
Cross-reactivity data - Panbio Dengue IgG Capture ELISA2
Disease type Total specimens Positive (or equivocal) result
West Nile virus 10 0/10
Malaria 10 0/10
Scrub typhus 7 0/7
Leptospira 12 2/12
Hepatitis A 6 0/6
Rheumatoid factor 10 0/10
Anti-nuclear antibody 10 1/10
TOTAL 65 3/65
Publications
Cuzzubbo, A., et al. (1997). Commercial Assays for the Serological Diagnosis of Dengue Infection. Arbovirus Research in Australia. 7:56-60. Queensland Institute of Medical Research.

Cuzzubbo, A., et al (1998) Detection of Specific Antibodies in Saliva during Dengue Infection. Journal of Clinical Microbiology Dec. 1998:3737-3739

Cuzzubbo, A., et al. (1999) Comparison of PanBio Dengue Duo Enzyme-Linked Immunosorbent
Assay (ELISA) and MRL Dengue Fever Virus Immunoglobulin M Capture ELISA for Diagnosis of Dengue Virus Infections in Southeast Asia. Clinical and Diagnostic Laboratory Immunology Sep. 1999:705-712

Porter, K., et al (1999) Evaluation of a Commercially Available Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay Kit for Diagnosing Acute Dengue Infections. Clinical and Diagnostic Laboratory Immunology Sep. 1999:741-744

Sang, C., et al (1998) Evaluation of a Commercial Capture Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin M and G Antibodies Produced during Dengue Infection. Clinical and Diagnostic Laboratory Immunology Jan. 1998: 7-10

Vaughn, D., et al (1999) RAPID SEROLOGIC DIAGNOSIS OF DENGUE VIRUS INFECTION USING A COMMERCIAL CAPTURE ELISA THAT DISTINGUISHES PRIMARY AND SECONDARY INFECTIONS. Am. J. Trop. Med. Hyg. 60(4): 693-698

Dengue IgG Capture ELISA
E-DEN02G
Please note these products are not for sale or distribution in the United States and may not be available in all countries worldwide. Please contact Inverness Medical or your local Panbio distributor to determine availability.


Product Overview
The Panbio Dengue IgG Capture ELISA is for the qualitative detection of IgG antibodies to dengue virus (serotypes 1, 2, 3 and 4) in serum. This test is intended as an aid in the clinical laboratory diagnosis of secondary dengue virus infection, and can be used in conjunction with the Panbio Dengue IgM Capture ELISA for the presumptive differentiation between primary and secondary infection.

The Panbio Dengue IgG Capture ELISA offers an alternative to the HAI assay as an aid in the serological diagnosis of secondary dengue infections.

The IgG cut-off is set to detect the high levels of IgG present in secondary infection.

As an aid in the diagnosis of secondary dengue
For use in endemic dengue regions
Use with the Dengue IgM Capture ELISA to detect and distinguish primary and secondary dengue with a high degree of confidence.
Quick Info
Excellent performance
Standardised method
Total incubation time 2 hr 10 min
Break-apart wells
Ready-to-use colour coded reagents
Compatible with standard microplate technology
Use in conjunction with E-DEN01M to differentiate primary and secondary infection
96 tests
Performance
Dengue IgM Capture and Dengue IgG Capture ELISA serological sensitivity and specificity1
Serological sensitivity IgM 95% CI* IgG 95% CI*
Primary dengue
54/57 (94.7%)
85.4-98.91%
N/A
N/A
Secondary dengue2 64/115 (55.7%)3 46.6-64.7% 52/54 (96.3%) 73.8-93.6%
Serological specificity IgM 95% CI* IgG 95% CI
Negatives
83/83 (100%)
95.7 - 100 % 125/125 (100%) 97.1-100%
Primary dengue N/A 95.7 - 100 % 32/35 (91.4%) 76.9-98.2%
This data has been extracted from the Dengue IgM Capture and Dengue IgG Capture ELISA Instructions for Use.
Samples positive for secondary dengue infection by HAI (titre >= 2560)
The use of the Panbio Dengue IgG Capture ELISA (E-DEN02G) is recommended when secondary dengue is suspected
* Confidence Interval
Cross-reactivity data - Panbio Dengue IgG Capture ELISA1
Disease type Total specimens Positive
West Nile virus 10 0/10
Malaria 10 0/10
Scrub typhus 7 0/7
Leptospira 12 2/12
Hepatitis A 6 0/6
Rheumatoid factor 10 0/10
Anti-nuclear antibody 10 1/10
TOTAL 65 3/65
Publications
Cuzzubbo, A., et al. (1997). Commercial Assays for the Serological Diagnosis of Dengue Infection. Arbovirus Research in Australia. 7:56-60. Queensland Institute of Medical Research.

Cuzzubbo, A., et al (1998) Detection of Specific Antibodies in Saliva during Dengue Infection. Journal of Clinical Microbiology Dec. 1998:3737-3739

Cuzzubbo, A., et al. (1999) Comparison of PanBio Dengue Duo Enzyme-Linked Immunosorbent Assay (ELISA) and MRL Dengue Fever Virus Immunoglobulin M Capture ELISA for Diagnosis of Dengue Virus Infections in Southeast Asia. Clinical and Diagnostic Laboratory Immunology Sep. 1999:705-712

Lam, K., et al (2000) Evaluation of a Capture Screening Enzyme-Linked Immunosorbent Assay for Combined Determination of Immunoglobulin M and G Antibodies Produced during Dengue Infection. Clinical and Diagnostic Laboratory Immunology Sep. 2000:850-852

Porter, K., et al (1999) Evaluation of a Commercially Available Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay Kit for Diagnosing Acute Dengue Infections. Clinical and Diagnostic Laboratory Immunology Sep. 1999:741-744

Sang, C., et al (1998) Evaluation of a Commercial Capture Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin M and G Antibodies Produced during Dengue Infection. Clinical and Diagnostic Laboratory Immunology Jan. 1998: 7-10

Vaughn, D., et al (1999) RAPID SEROLOGIC DIAGNOSIS OF DENGUE VIRUS INFECTION USING A COMMERCIAL CAPTURE ELISA THAT DISTINGUISHES PRIMARY AND SECONDARY INFECTIONS. Am. J. Trop. Med. Hyg. 60(4): 693-698

Vazquez, S., et al (2007) Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections. Journal of Clinical Virology 39:194-198

Instructions for use

Dengue IgM Capture ELISA
E-DEN01M
Please note these products are not for sale or distribution in the United States and may not be available in all countries worldwide. Please contact Inverness Medical or your local Panbio distributor to determine availability.

Dengue Early ELISA
E-DEN01P
Please note these products are not for sale or distribution in the United States and may not be available in all countries worldwide. Please contact Inverness Medical or your local Panbio distributor to determine availability.


Product Overview
PLEASE NOTE: This product will be discontinued and will be replaced with the second generation Dengue Early ELISA (E-DEN02P)

The Panbio Dengue Early ELISA is a dengue NS1 antigen capture ELISA. It is for the qualitative detection of NS1 antigen (serotypes 1, 2, 3 and 4) in serum and used as an aid in the clinical laboratory diagnosis of patients with clinical symptoms consistent with dengue fever.

The Dengue Early ELISA should be used in conjunction with other dengue serological assays.

As an aid in early diagnosis of acute dengue infection
Suitable for use in endemic and non-endemic dengue regions
Recommended for use with Dengue Duo IgM Capture and IgG Capture ELISA as an aid in delivering a more comprehensive diagnosis of dengue infection in endemic regions or in regions where secondary dengue is prevalent.
Quick Info
Excellent performance (90% sensitivity, 100% specificity)
Standardised method
Short assay time (2 hr 10 min)
Break-apart wells
Ready-to-use colour coded reagents
Compatible with standard microplate technology
96 tests
Publications
Blacksell, S. et al (2007) Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. Diagnostic Microbiology and Infectious Disease 60:43-49

Devi, S,. et al (2007) Evaluation of a Dengue NS1 capture ELISA assay for the rapid detection of Dengue. J. Infect Developing Countries. 1(2)L182-188

Simmons, C., et al (2007) Maternal Antibody and Viral Factors in the Pathogenesis of Dengue Virus in Infants. Journal of Infectious Diseases. 196:416-24

Instructions for use

Rabbit polyclonal to LOC541473 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to LOC541473 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to LOC541473 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to PRY2 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to ZNF772 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Rabbit polyclonal to SNX19 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to KRT39 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to ZCCHC13 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to MMAB - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to EPHA6 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to ZDHHC23 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to CTAG1A - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Rabbit polyclonal to RFX6 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Rabbit polyclonal to ATOH7 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to UNC13D - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to DZIP1L - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to DCP1B - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to CALML6 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to ZNF296 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Rabbit polyclonal to AMOTL1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to RP1-127L4.6 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to HSCB - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to LIPI - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to C20orf186 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to WFDC5 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to NRG4 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to GLIPR1L2 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to PIWIL4 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to MPP7 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to TRIM69 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to CD109 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Rabbit polyclonal to IQGAP3 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to B3GALT6 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to WTIP - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to ZSWIM7 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to WFIKKN2 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to ZC3H18 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to PCMTD1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to TMEM123 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to STRADA - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to C9orf30 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to LHX4 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Rabbit polyclonal to FGD3 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to LMLN - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to ZCRB1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to RHPN2 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to MYLK2 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to GTPBP3 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to TRIM63 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Rabbit polyclonal to PGBD1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to FAM120B - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to ANKRD20A1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to MYST1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to PRAM1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to HDAC10 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to KLF16 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to ARMC10 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to RIOK1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to COG3 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to MXD3 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Rabbit polyclonal to DIAPH3 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to PDRG1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to CFHR5 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to DDHD1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to ZKSCAN3 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to PLEKHO2 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to HKDC1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to MUS81 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to MRM1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to MMRN2 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to THAP9 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to FLJ22167 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to GCC1 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to MMP28 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to CARD14 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to TMEM38A - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Rabbit polyclonal to FUNDC2 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to FUNDC2 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to FUNDC2 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Rabbit polyclonal to ZSCAN18 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Rabbit polyclonal to SLC26A6 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Mouse polyclonal to AIDA - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.